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protein lysate  (Jena Bioscience)


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    Structured Review

    Jena Bioscience protein lysate
    Protein Lysate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5+azide/Cy5%2E5-Azide/pmc13096800-47-10-3
    Average 94 stars, based on 8 article reviews
    protein lysate - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Live imaging of human airway epithelial repair in precision-cut lung slices after targeted cell damage
    Article Snippet: Cell permeabilization was conducted by incubation in 0.3 % Triton X-100 (Carl Roth, Germany) in DPBS for 2 h on a shaker at 130 rpm and RT. .. A subsequent click-chemistry-mediated staining reaction was achieved by incubation of hPCLS in the reaction mix (1 mM Cu2SO4 (Jena Bioscience, Germany), 10 mM sodium ascorbate (Jena Bioscience, Germany), 80 nM Cy5 azide (Jena Bioscience, Germany) diluted in DPBS (Sigma-Aldrich, MO, United States)) for 1 h at 37 °C in the dark, leading to fluorescent visualization of EdU that was incorporated into the DNA. ..

    Incubation:

    Article Title: Live imaging of human airway epithelial repair in precision-cut lung slices after targeted cell damage
    Article Snippet: Cell permeabilization was conducted by incubation in 0.3 % Triton X-100 (Carl Roth, Germany) in DPBS for 2 h on a shaker at 130 rpm and RT. .. A subsequent click-chemistry-mediated staining reaction was achieved by incubation of hPCLS in the reaction mix (1 mM Cu2SO4 (Jena Bioscience, Germany), 10 mM sodium ascorbate (Jena Bioscience, Germany), 80 nM Cy5 azide (Jena Bioscience, Germany) diluted in DPBS (Sigma-Aldrich, MO, United States)) for 1 h at 37 °C in the dark, leading to fluorescent visualization of EdU that was incorporated into the DNA. ..

    other:

    Article Title: Metamorphic development of the olfactory system in the red flour beetle ( Tribolium castaneum , H erbst )
    Article Snippet: Cy5-Sulfo Azide , Cy5-azide , , 1 μM , Jena Bioscience, Jena, Germany (CLK-AZ118-1; Kli009-030; n/a) , , .

    Purification:

    Article Title: RNA-associated glycoconjugates highlight potential ambiguities in glycoRNA analysis
    Article Snippet: The resulted reaction mixture was then purified using TRIzol in combination with Zymo RCC-25 following the vendor-provided protocol. .. The purified DBCO-functionalized RNA (10 μg per reaction) was then clicked with either Cy5-azide (Jena Bioscience, CLK-1177) or the AlexaFluor-555-labeled sialoglycan-azide at 200 and 60 μM, respectively, at 50 °C for 10 min. .. The clicked RNA product was then purified with TRIzol in combination with Zymo RCC-10 following the vendor’s protocol.

    Concentration Assay:

    Article Title: Nucleotidyl transferase assisted DNA labeling with different click chemistries
    Article Snippet: For CuAAC of alkyne-containing DNA biotin azide or Alexa Fluor 647 azide (250 μM; Life Technologies) was added to purified DNA (250 nM) tailed with alkyne-containing nucleotides (TdT-reaction: 1 μM DNA, 10 μM 5- E -UTP, 5- O -dUTP, or N 6 - P -ATP, or 100 μM 5- E -dUTP). .. Alternatively, Cy5 azide (500 μM; Jena Bioscience) was added to non-purified TdT mixture (final concentration in CuAAC: 250 nM DNA, 2.5 μM 5- O -dUTP). ..



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